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Molecular evidence for Wolbachia infection in Prorops nasuta (Hymenoptera: Bethylidae), a parasitoid wasp of the coffee berry borer Evidencia molecular de infección por Wolbachia en Prorops nasuta (Hymenoptera: Bethylidae), una avispa parasitoide de la broca del café.

How to Cite
Navarro, L., Maldonado, C. E., & Benavides, P. (2019). Molecular evidence for Wolbachia infection in Prorops nasuta (Hymenoptera: Bethylidae), a parasitoid wasp of the coffee berry borer. Cenicafe Journal, 70(2), 45-54. https://doi.org/10.38141/10778/70204

Dimensions
PlumX

Keywords
Endosymbiont

biological control

Hypothenemus hampei

Endosimbionte

control biológico

Hypothenemus hampei

Sectión
Articles
Lucio Navarro
Carlos Ernesto Maldonado
Pablo Benavides

Summary

Wolbachia are common endosymbiotic bacteria of insects known for manipulating host reproduction in different ways. In this study we report Wolbachia infection in the parasitoid wasp Prorops nasuta, an insect introduced into the Americas for the biological control of the coffee berry borer Hypothenemus hampei. PCR screening for Wolbachia infection based on the endosymbiont marker genes fstZ and wsp resulted positive in wasps samples collected in coffee fields from Colombia and Brazil and obtained from a laboratory colony in Mexico. DNA sequence analyses of the wsp gene identified two different sequence clones that suggest multiple Wolbachia isolates infecting these wasp populations. Additionally, phylogenetic analysis of the ftsZ and wsp sequences located these Wolbachia isolates into supergroup A. Whether Wolbachia plays a role affecting reproduction in P. nasuta remains unclear. However, different scenarios of how Wolbachia-infection outcomes may affect insect-pest control using parasitoids are discussed.

Lucio Navarro, Centro Nacional de Investigaciones de Café

Investigador Científico I, Disciplina de Entomología. Cenicafé.


Carlos Ernesto Maldonado, Centro Nacional de Investigaciones de Café

Investigador Científico I, Disciplina de Mejoramiento Genético. Cenicafé.


Pablo Benavides, Centro Nacional de Investigaciones de Café

Investigador Científico III, Disciplina de Entomología. Cenicafé.


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