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Coffee crop demands phosphorus (P) during the nursery stage and vegetative growth. These requirements are commonly satisfied by applying a high dose of soluble phosphatic fertilizers, which increases production costs and causes environmental concerns. The Colombian coffee zone has a great diversity of soils with variable mineral composition and organic matter (OM) content. These conditions exhibit a long-term stability, concomitantly govern soil P availability and, hence, a standard recommendation for P fertilization may cause nutritional imbalance in the soil solution. To accurately estimate soil P-availability in Colombian coffee soils, a P-fixation (PF)-based approach using sorption isotherms technique was evaluated in five Andisols and four Inceptisols. Changes in the soil solution P concentration were monitored over time. Results showed that PF value was overestimated (> 68 %) during 36 h of equilibration; after this period, the system stabilized, providing reliable PF values. The relation between PF and applied P was assessed at a concentration of 0.2 mg L-1 (P0.2), level considered adequate in the soil solution to support maximal crop yields in different cultivated plants, was achieved by applications between 104 and 773 mg kg-1 of P in Andisols, and between 0 and 349 mg kg-1 of P in Inceptisols. OM content (OM: 7.7%), presence of non-crystalline materials, texture, and mineralogy explained the PF across soils. These finding suggest that a PF-based approach could serve as a valuable tool for developing site-specific P fertilization strategies and useful for establishing future P coffee fertilization in Colombia.