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Nitrogen-N has been pointed out as the most limiting nutrient for coffee production in Colombia. This research aimed to evaluate the application of spectral indexes to determine the needs of N in coffee (Coffea arabica L.) during the production stage. The experiment was conducted at the Experimental Farm Paraguaicito (Buenavista, Quindío), where five urea N doses (0, 150, 225, 300 and 375 kg·ha-1·year-1) were applied in April and October from 2013 to 2015, the treatments were assigned under a randomized complete blocks design with four replications. Before administering the treatments, the relative chlorophyll-IRC index was evaluated in each season with the SPAD-502 equipment in the lower, middle and upper thirds of the plants. Likewise, the Normalized Differential Vegetation Index-NDVI was evaluated with the GreenSeeker equipment and the annual coffee berry yield was recorded. The SPAD readings did not allow to accurately determine the need to make adjustments to the nitrogen fertilization of coffee, the average values of NDVI were reduced with the increase in the age of the crop by expressing the vigor of the plants, while the accumulated production responded to the increasing doses of N with a linear trend (P <0.0001).